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采用形态判别、同工酶分析和RAPD分析相结合的方法 ,从 3个层次研究分析了鲂属团头鲂、三角鲂和广东鲂的种间亲缘关系和种内遗传差异。结果表明 :(1) 3种鲂在形态可数性状上差异不显著 ,而可量性状与框架分析揭示团头鲂与三角鲂亲缘关系较近 ,它们同广东鲂差异较大 ;(2 )团头鲂和三角鲂均具有MDH同工酶的s Mdh D位点 ,而广东鲂未见 ,引物S11扩增的结果在 3种鲂间均显示种的特异性 ,这些同工酶谱带和DNA扩增带可作为 3种鲂的种间分子标记 ;(3) 3种鲂种间亲缘关系在三个研究层面上相互吻合 :即广东鲂与团头鲂、三角鲂差异较大 ,亲缘关系较远 ,而三角鲂和团头鲂之间差异小 ,亲缘关系较近 ;(4 )同工酶和RAPD分析揭示 ,三角鲂种内遗传多样性显著地高于广东鲂和团头鲂。
Using the methods of morphological discrimination, isoenzyme analysis and RAPD analysis, the genetic relationship and the intraspecific genetic differences among species of Tamarix ramosissima, Tridacna scoparia and Tamarix ramosissima were studied from three levels. The results showed that: (1) There was no significant difference in morphological traits among the three species, but the quantitative traits and framework analysis revealed that the genetic relationship between the two species was relatively close, and the differences between them were quite different from those in Guangdong Province. (2) Both 头 鲂 and 三 三 all had MDH isozymes at MdhD locus, whereas 广 鲂 had not been seen in Guangdong,. The result of primer S11 amplification showed species specificity in all three species. The isozyme bands and DNA The amplified bands could be used as the interspecific molecular markers for the three species. (3) The genetic relationship among the three species was consistent with each other at three research levels: that is, there were significant differences between the two species (4) Isoenzyme and RAPD analysis revealed that the genetic diversity within the Triangle Brachycephalus was significantly higher than that of the Brahmaputra in Guangdong.