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应用化学发光方法研究了4种儿茶素及其组合物对超氧阴离子自由基(O·2-)和羟自由基(OH·)的清除能力,并对其之间的清除能力进行了比较。结果表明:EGCG、ECG、EGC和EC的O·2-清除率为50%时的浓度SC50分别为4.2、4.9、5.2和6.2μmol/L,而OH·清除率为50%时的浓度SC50则分别为0.22、0.25、0.26和0.30μmol/L。4种儿茶素的浓度相同时,其O·2-和OH·清除率的大小顺序为:EGCG>ECG>EGC>EC。并应用正交试验设计方法来优化4种儿茶素的组合,结果表明:清除O·2-和OH·的最佳组合是EGCG∶ECG∶EGC∶EC为3∶3∶1∶1。EGCG对自由基清除率影响是最大和最显著的。在4种儿茶素的组合中,当EGCG所占的比例较大时,其组合物的自由基清除率比单体的自由基清除率要大。文中最后还模拟了不同茶叶中4种儿茶素所占的比例不同时,其清除自由基的能力的大小。本研究为利用儿茶素的不同配伍寻找一种更能发挥其药用功效的复合物提供了一条新的途经。
The chemiluminescence method was used to study the scavenging ability of four catechins and their compositions on O · 2 and OH ·, and their scavenging capacities were compared . The results showed that the concentrations of SC50 at EGCG, ECG, EGC and EC at O · 2- clearance of 50% were 4.2, 4.9, 5.2 and 6.2 μmol / L, respectively, while those at OH · scavenging rate of 50% 0.22, 0.25, 0.26 and 0.30 μmol / L, respectively. When the concentrations of 4 kinds of catechins were the same, the order of O · 2- and OH · clearance was EGCG> ECG> EGC> EC. Orthogonal design method was used to optimize the combination of four catechins. The results showed that the optimum combination of O · 2- and OH · was EGCG: ECG: EGC: EC of 3: 3:1:1. EGCG on free radical scavenging rate is the largest and most significant. Among the four catechin combinations, the free radical scavenging rate of the composition was larger than that of the monomer when the proportion of EGCG was large. Finally, the paper also simulated the different tea catechins in four different proportion of the time, its ability to scavenge the size of free radicals. This study provides a new way to find a compound that can exert its medicinal efficacy by using the different compatibility of catechins.