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目的:探讨黄连、五倍子醇提物对罗非鱼源性链球菌抑制活性。方法:采用BHI(脑心浸液肉汤)固体培养基琼脂稀释法分别检测黄连、五倍子醇提物对链球菌的最小抑菌浓度(MIC)和最小杀菌浓度(MBC);选取五倍子生药质量浓度MIC(3.125 g.L-1)为该实验的低浓度、2倍MIC为中质量浓度(6.25 g.L-1)、3倍MIC为高质量浓度(9.375 g.L-1)。黄连生药质量浓度MIC(13.33 g.L-1)为低浓度、MBC(20.00 g.L-1)为中浓度、2倍MIC(26.67 g.L-1)为高浓度。挑取链球菌单菌落于各组中,搅拌后,于37℃200 r.min-1的条件下培养,于0.5,1.5,2.5,3.5,4.5,5.5,6.5,7.5,8.5 h取样,计算菌落数,并绘制体外杀菌曲线,观察低、中、高浓度下黄连和五倍子醇提物链球菌的动态杀菌过程。结果:五倍子醇提物低浓度和中浓度、高浓度分别在2.5,0.5 h出现抑菌作用。高、中、低浓度分别在6.5,8.5,8.5 h达到杀菌效果。黄连醇提物各浓度在0.5 h出现抑菌作用。高、中,低浓度分别在2.5,4.5,4.5 h达到杀菌效果。结论:黄连和五倍子均有较强的体外抑菌杀菌作用,特别是黄连,使无乳链球菌无正常生长周期,直接进入衰竭期,达到杀菌效果。
Objective: To investigate the inhibitory activity of alcohol extract of berberine and gallnut on Streptococcus tilapia. Methods: The minimal inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of Streptococcus were tested by BHI (Brain Heart Infusion Broth) solid medium agar dilution method. MIC (3.125 gL-1) was the low concentration for this experiment. The 2-fold MIC was medium concentration (6.25 gL-1) and the 3-fold MIC was high concentration (9.375 gL-1). The concentration of MIC was 13.33 g.L-1, MBC was 20.00 g.L-1 and the concentration of MIC was 26.67 g.L-1. Strains of single streptococci were picked into each group. After stirring, they were cultured under the condition of 200 r.min-1 at 37 ℃ and sampled at 0.5, 1.5, 2.5, 3.5, 4.5, 5.5, 6.5, 7.5 and 8.5 h The number of colonies was plotted and the bactericidal curves in vitro were drawn. The dynamic sterilization process of Streptococcus pyogenes with Coptis chinensis and Galla chinensis under low, medium and high concentrations was observed. Results: The antibacterial activity of gallnut alcohol extract at low concentration, medium concentration and high concentration were 2.5 and 0.5 h, respectively. High, medium and low concentrations of 6.5, 8.5, 8.5 h bactericidal effect. Rhizoma alcohol extract concentrations in 0.5 h antibacterial effect. High, medium and low concentrations of 2.5,4.5,4.5 h bactericidal effect. Conclusion: Coptis chinensis and Chinese nutgall have strong antibacterial and bactericidal activity in vitro, especially Coptis, so that Streptococcus agalactiae has no normal growth cycle, directly enter the failure phase to achieve the bactericidal effect.