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采用日立Z 80 0 0原子吸收分光光度计测Na+ 、K+ 含量 ,采用不连续蔗糖梯度离心分离质膜和液泡膜微囊。递增盐度和盐冲击处理下 ,耐盐品种德抗 96 1的SK ,Na(吸收 ) 值和SK ,Na(运输 ) 值均明显大于盐敏感品种鲁麦 15 ;德抗 96 1根部和鲁麦 15根茎结合部Na+ 含量均呈递增趋势 ,表现出累积效应 ;德抗 96 1根细胞质膜微囊和液泡膜微囊H+ ATP酶活性均明显大于鲁麦15 ,鲁麦 15根茎结合部液泡膜微囊H+ ATP酶活性大于德抗 96 1,在同一品种的植株里 ,盐冲击的根和根茎结合部细胞质膜微囊和液泡膜微囊H+ ATP酶活性均小于递增盐度的酶活性。小麦拒Na+ 部位细胞质膜和液泡膜H+ ATP酶活性与其耐盐性强弱成正相关
Hitachi Z 80 0 0 atomic absorption spectrophotometer Na +, K + content, the use of discontinuous sucrose gradient centrifugation plasma membrane and vacuolar microcapsules. Under the condition of increasing salinity and salt shock, the SK, Na (absorption) values and SK and Na (transport) values of the salt-tolerant cultivar Duroc 961 were significantly higher than those of the salt-sensitive cultivar Lumai 15; The content of Na + in the rhizome binding sites showed an increasing trend and showed a cumulative effect. The activities of H + ATPase in plasma membrane and vacuolar membrane microcapsules of DEK-96 were significantly higher than those of the control The activity of H + -ATPase in cytoplasm was higher than that in DE 96. In the same variety of plants, the activity of cytoplasmic membrane microcapsules and vacuolar membrane H + -ATPase in roots and rhizome-binding cells under salt shock were less than those of increasing salinity. There was a positive correlation between the activities of plasma membrane and tonoplast H + ATPase in Na + -releted wheat lines and their salt tolerance