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番茄果实的成熟是由多基因精细调控的一个过程。其中作为重要的植物激素,乙烯和ABA又与成熟过程密切相关。在乙烯合成途径中,ACS和ACO两个基因家族起着非常重要的作用。而ABA与乙烯在植物成熟衰老过程中的相互作用也开始成为研究热点。采用真空渗透技术对不同时期的番茄果实(未熟、绿熟、破色)进行ABA渗透处理。然后根据处理4h(4hpi)和24h(24hpi)后的果实乙烯生成情况,确定处理后4h为基因表达测定时间。最后利用实时定量PCR技术,对3个不同成熟时期番茄果实中的ACS(LeACS1A,LeACS2,LeACS4和LeACS6)和ACO(LeACO1,LeACO3,LeACO4和LeACO6)进行基因表达测定。
Tomato fruit maturation is a process regulated by multiple genes. Among them, ethylene and ABA, as important plant hormones, are closely related to maturation process. In the ethylene synthesis pathway, ACS and ACO two gene families play a very important role. The interaction between ABA and ethylene in plant maturation and senescence has also become a research hotspot. The infiltration of ABA was carried out on tomato fruits (immature, green and broken) in different periods by vacuum infiltration technique. Then according to the fruit ethylene production after 4h (4hpi) and 24h (24hpi) treatment, the determination of gene expression time 4h after treatment. Finally, real-time quantitative PCR was used to determine the gene expression of ACS (LeACS1A, LeACS2, LeACS4 and LeACS6) and ACO (LeACO1, LeACO3, LeACO4 and LeACO6) in tomato fruit at three different ripening stages.