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目的 研究不同品牌IFNα2a或IFNα2b的免疫调节作用。方法 采用MTT法进行NK细胞的细胞毒试验和用流式细胞仪测定淋巴细胞CD抗原表达。结果 2 0 0IU/ml不同品牌的IFNα2a或IFNα2b能促进NK细胞杀伤K562 细胞 ,其杀伤效率可达 35 %左右 ,比不加干扰素对照组NK细胞杀伤效率约 14%均提高了近 2 .5倍 (P <0 .0 5 ) ,可使CD4 + /CD8+ 的比值升高 (P <0 .0 5 )。结论 进口和国产的不同品牌IFNα2a和IFNα2b具有同等的免疫调节功效。
Objective To study the immunomodulatory effects of different brands of IFNα2a or IFNα2b. Methods The cytotoxicity of NK cells was measured by MTT assay and CD antigen expression of lymphocytes was determined by flow cytometry. Results Different brands of IFNα2a or IFNα2b could promote NK cell killing K562 cells with the killing efficiency of about 35%, which was nearly 2.5% higher than that of the control group with interferon-free NK cells of about 14% (P <0.05), which could increase the ratio of CD4 + / CD8 + (P <0.05). Conclusion The imported and domestic brands IFNα2a and IFNα2b have equivalent immunomodulatory effects.