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以玉米高密度遗传连锁图谱IBM2 2008 Neighbors为参考图谱,收集来自不同实验中的175个玉米磷素相关根系数量性状位点(QTL)信息,利用Biomercator 2.1软件,构建玉米磷相关根系QTL整合图谱。采用元分析技术,在10条染色体上发掘出26个与磷相关根系性状“一致性”QTL(MQTL),图距范围在0.8~14.3 c M之间,除第2、5、8号染色体外,其余染色体上均检测到2~4个MQTL。根据MQTL区间两端标记在玉米物理图谱Ref Gen_v2上的位置,将MQTL进行物理图谱定位,单个MQTL所在物理图谱上的图距范围在0.1~9.1 Mb。在MQTL 1、2、4、8、11、12、15、18、20、22中检测到15个与磷素吸收利用以及根系性状的候选基因。
Based on the high-density maize genetic linkage map IBM2 2008 Neighbors, 175 quantitative trait loci (QTLs) related to phosphorus-related roots in maize were collected from different experiments. Biotercator 2.1 software was used to construct the QTL mapping map of maize related phosphorus. Using elemental analysis, 26 QTLs associated with phosphorus-related root traits (QTLs) were mapped on 10 chromosomes with a range of 0.8 to 14.3 cM. Chromosomes, the remaining chromosomes were detected 2 to 4 MQTL. According to the positions of markers located on the Ref Gen_v2 map of maize at both ends of the MQTL interval, the physical map of the MQTL is located. The range of the physical map of a single MQTL is between 0.1 and 9.1 Mb. Fifteen candidate genes for phosphorus uptake and utilization and root traits were detected in MQTL 1, 2, 4, 8, 11, 12, 15, 18,