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用高效液相色谱法 (HPLC)测定了鞭角华扁叶蜂 (Chinolydaflagellicornis)末龄幼虫及滞育预蛹血淋巴中保幼激素 Ⅲ (JHⅢ )的滴度。结果表明 ,末龄幼虫血淋巴中JHⅢ的滴度虽呈明显下降趋势 ,但仍保持较高的水平(17ng·mL- 1 ) ;滞育预蛹血淋巴中JHⅢ的滴度达 2 0ng·mL- 1 左右 ,而在化蛹前一天则可达 30ng·mL- 1 以上。表明JH对鞭角华扁叶蜂预蛹滞育起调节作用。
The titers of juvenile hormone (JHⅢ) in the late stage of larvae of Chinolydaflagellicornis and in the hemolymph pre-pupal hemolymph were measured by high performance liquid chromatography (HPLC). The results showed that although the titer of JHIII in the hemolymph of the late-stage larvae was significantly decreased, but remained high (17ng · mL-1); the titers of JHIII in the hemolymph pre-pupal haemolymph reached 20 ng · mL - 1 or so, but up to 30 ng · mL-1 on the day before pupation. It showed that JH played a regulatory role in the pre-pupal diapause of Hyphantria cunea.