论文部分内容阅读
目的:观察恶性肿瘤模型小鼠和抑郁模型小鼠行为学差异,以及血府逐瘀口服液对其的影响,并探究其作用机理。方法:将雄性昆明小鼠随机分为7组,正常对照组8只,抑郁模型组、抑郁血府逐瘀组、抑郁黛力新组、肿瘤模型组、肿瘤血府逐瘀组、肿瘤黛力新组,每组各7只。抑郁小鼠模型采用慢性轻度不可预见性的应激法建立。肿瘤造模选用腹水型S180肉瘤小鼠皮下接种。两血府逐瘀组和两黛力新组小鼠分别给予血府逐瘀口服液、黛力新片灌胃干预治疗21 d。观察治疗前后小鼠行为学改变,治疗后5-HT含量,肿瘤小鼠的瘤重。结果:经过21 d的干预,与正常对照组及抑郁两灌胃组相比,抑郁模型组及肿瘤模型组小鼠糖水消耗量明显减少(P<0.01),垂直运动及水平运动活动度明显下降,且血府逐瘀组稍优于黛力新组(P<0.05);与肿瘤模型组相比,肿瘤血府逐瘀组、肿瘤黛力新组糖水消耗量明显增加(P<0.01);两种干预药物作用相似。在5-HT方面,正常对照组、抑郁血府逐瘀组含量较高,与其它各组比较差异具有统计学意义(P<0.05)。肿瘤血府逐瘀组瘤体较肿瘤模型组及肿瘤黛力新组明显缩小(P<0.01)。结论:应激及肿瘤可以导致实验小鼠出现行为学改变和5-HT含量降低的抑郁表现;血府逐瘀口服液能有效干预行为学改变和5-HT含量的降低,并能抑制肿瘤生长,作用优于黛力新片。
OBJECTIVE: To observe the difference of behavior between mice model of malignant tumor and depression model and the effect of Xuefuzhuyu Oral Liquid, and to explore its mechanism. Methods: Male Kunming mice were randomly divided into 7 groups: normal control group (n = 8), depression model group, depression Xuefu Zhuyu group, Depression nataloxin group, tumor model group, tumor Xuefu Zhuyu group, New group, each group of 7. Depression mouse model using chronic mild unpredictable stress method to establish. Selection of ascites tumor model S180 sarcoma mice subcutaneously inoculated. Two Xuefuzhuyu group and two Deanxit mice were given Xuefuzhuyu oral liquid, Deanin tablets for gastric intervention 21d. Observed before and after treatment mice behavioral changes, 5-HT content after treatment, tumor weight of tumor mice. Results: Compared with the normal control group and the depression-fed group, the consumption of syrup in the depression model group and the tumor model group decreased significantly (P <0.01) after 21 days of intervention, and the vertical movement and horizontal exercise activity decreased significantly , And Xuefu Zhuyu group was slightly better than Deanxit group (P <0.05). Compared with tumor model group, Xuefuzhuyu group and Dexamethoxine group showed a significant increase in sugar consumption (P <0.01); The two interventions have similar effects. In the aspect of 5-HT, the levels in normal control group and Xuefu Zhuyu group were higher than those in other groups (P <0.05). The tumor of Xuefuzhuyu group was significantly smaller than that of tumor model group and dexamethasone group (P <0.01). Conclusion: Stress and tumor can lead to behavioral changes and depression of 5-HT in experimental mice. Xuefuzhuyu Oral Liquid can effectively change the behavioral changes and reduce the content of 5-HT, and can inhibit tumor growth , The effect is better than Deanxit film.