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目的:采用免疫亲和柱净化,结合柱后光化学衍生化的高效液相色谱-荧光检测器建立同步检测常用中药材及染菌中药制剂中间体中的黄曲霉毒素B1,B2,G1,G2的方法。方法:采用免疫亲和柱净化洗脱结合柱后光化学衍生手段,建立黄曲霉毒素B1,B2,G1,G2的分析方法,并分别对14种中药材及染菌中药制剂中间体进行检测。结果:黄曲霉毒素B2和G2,B1和G1分别在0.15～6.0和0.5～20.0 ng·mL-1线性关系良好,方法准确稳定。所选的14种中药中川芎和柏子仁检测出黄曲霉毒素B1,而以染菌中药川芎所制备的5种提取物中均未检出黄曲霉毒素。结论:采用此方法检测常用中药材及其制剂中间体中的黄曲霉毒素无干扰性杂峰,结果准确可靠。
OBJECTIVE: To establish simultaneous detection of aflatoxins B1, B2, G1 and G2 in traditional Chinese medicinal materials and intermediates of traditional Chinese medicine preparation by immunoaffinity column purification and column photochemical derivatization with high performance liquid chromatography-fluorescence detector method. Methods: The analytical method of aflatoxins B1, B2, G1 and G2 was established by means of immunoaffinity column purification combined with photochemical derivatization. The intermediates of 14 kinds of Chinese medicinal materials and traditional Chinese medicine preparations were tested respectively. Results: The linear relationship between aflatoxins B2 and G2, B1 and G1 was between 0.15-6.0 and 0.5-20.0 ng · mL-1, respectively. The method was accurate and stable. The selected 14 kinds of Chinese medicine Chuanxiong and Bozi were detected aflatoxin B1, and the bacteria extract Chuanxiong 5 extracts were not detected aflatoxin. Conclusion: This method is applicable to the detection of aflatoxins in common Chinese herbal medicines and their intermediates. The results are accurate and reliable.