论文部分内容阅读
目的：获得高纯度的乳鼠雪旺氏细胞。方法：采用出生后３～５天乳鼠的坐骨、臂丛神经，植块法培养乳鼠雪旺氏细胞；并通过精细剥除神经外膜、组织块反复再植、低浓度胰酶快速消化、差速贴壁法的有机结合对雪旺氏细胞进行纯化；继用抗Ｓ－１００蛋白单抗通过间接免疫荧光法及ＡＢＣ法进行细胞鉴定。结果：首次植块获得的细胞经纯化后雪旺氏细胞可高达８５％以上，反复植块者可达９５％，甚至高达９８％。结论：本方法简单、稳定，所获得的雪旺氏细胞纯度高、数量大、受非实验因素影响小
OBJECTIVE: To obtain high purity rat Schwann cells. Methods: The Schwann cells were cultured in the islets of 3 to 5 days after birth in neonatal rats. The rats were sacrificed by extirpation of the epineurium. The tissues were repeatedly replanted and rapidly digested with low concentration of trypsin , Differential adherent method for the purification of Schwann cells with the organic combination; followed by anti-S-100 monoclonal antibody by indirect immunofluorescence and ABC method for cell identification. Results: The purified Schwann cells can reach more than 85% after the first explantation, and up to 95% or even up to 98% of the repeat explants. Conclusion: The method is simple and stable, and the obtained Schwann cells have high purity and large quantity, and are less affected by non-experimental factors