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目的:评价STAT3反义寡核苷酸对人肺腺癌细胞A549增殖抑制作用及辐射增敏作用,探讨新的肺癌分子靶向治疗药物。方法:2003-10/2004-11于解放军第二军医大学放射医学教研室,应用阳离子脂质体介导STAT3反义寡核苷酸转染人肺腺癌A549细胞,westernblot检测STAT3总蛋白和p-STAT3蛋白的表达;MTT法检测细胞增殖状态;流式细胞术检测细胞周期;用克隆形成分析和SF2研究人肺腺癌细胞A549辐射敏感性的变化。结果:STAT3反义寡核苷酸转染人肺腺癌A549后STAT3蛋白的表达和磷酸化水平下降,细胞增殖受抑制,出现G1阻滞;照射后12d,反义寡核苷酸组测定SF2值是(46.78%±3.25%),较对照组(61.52%±2.74%)明显降低(P<0.01)。结论:STAT3反义寡核苷酸可以抑制STAT3蛋白表达和细胞增殖,对人肺腺癌细胞A549有辐射增敏作用。
Objective: To evaluate the antitumor effect of STAT3 antisense oligonucleotide on the proliferation of human lung adenocarcinoma A549 cells and its radiosensitization, and to explore new molecular targeted drugs for lung cancer. METHODS: Human lung adenocarcinoma A549 cells were transfected with cationic liposome-mediated STAT3 antisense oligonucleotides from 2003-10 / 2004-11 at the Department of Radiation Medicine, Second Military Medical University of PLA. Western blot was used to detect STAT3 total protein and p- STAT3 protein expression; MTT assay cell proliferation status; flow cytometry cell cycle; clone formation analysis and SF2 human lung adenocarcinoma cell A549 radiation sensitivity changes. Results: After STAT3 antisense oligonucleotide was transfected into human lung adenocarcinoma A549, the expression and phosphorylation of STAT3 protein decreased and the cell proliferation was inhibited. The G1 arrest was observed. After antisense oligodeoxynucleotide assay, (46.78% ± 3.25%), which was significantly lower than that of the control group (61.52% ± 2.74%) (P <0.01). CONCLUSION: STAT3 antisense oligonucleotide can inhibit the expression of STAT3 protein and cell proliferation and sensitize human lung adenocarcinoma cell line A549 to radiation.