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为了掌握胞内劳森菌在湖北省5个疑似猪增生性肠炎暴发规模化猪场中的流行情况,我们建立了一种从粪便中检测胞内劳森菌的巢式PCR方法,并利用该方法对采集于这5个规模化猪场不同生产阶段不同临床表现猪群的1 003份粪便样品进行监测。结果显示:5个猪场的胞内劳森菌检出率在11.5%~19.4%之间,平均阳性率为15.5%;就临床表现而言,腹泻猪粪便样品中胞内劳森菌的检出率高于非腹泻猪;就生产阶段而言,保育及育肥猪中胞内劳森菌的检出率高于断奶仔猪、母猪和公猪。我们还利用粪便检测胞内劳森菌呈阳性、疑似临床症状明显的猪的肠道制备的感染溶液进行了病例复制,试验猪在灌服感染溶液后出现了与胞内劳森菌感染相似的临床症状及病理变化,并且从感染猪的肠道组织和粪便中检测到了胞内劳森菌的存在。本试验对于进一步了解胞内劳森菌在我国猪群中的流行情况及致病性具有一定的意义。
In order to understand the prevalence of Lawsonia intracellularis in five pig farms in Hubei Province that suspected swine plague outbreaks, we established a nested PCR method to detect Lawsonia intracellularis in feces, Methods A total of 1 003 stool samples collected from pigs with different clinical manifestations at different production stages of the five large-scale pig farms were monitored. The results showed that the detection rates of Lawsonia intracellularis in 5 pig farms ranged from 11.5% to 19.4% and the average positive rate was 15.5%. In terms of clinical manifestations, the detection of Lawsonia intracellularis in diarrhea pig stool samples Out rate than non-diarrhea pigs; in the production stage, the detection rate of intracellular Lawsonia in the conservation and finishing pigs was higher than that in weaned pigs, sows and boars. We also performed a case-by-case review of the gut-derived infectious solution of feces positive for Lawsonia intracellularis and suspected clinical symptoms in pigs that developed similar infection with Lawsonia intracellularis after inoculation of the infected solution Clinical symptoms and pathological changes, and the presence of Lawsonia intracellularis was detected from intestinal tissues and feces of infected pigs. This experiment is of great significance to further understand the prevalence and pathogenicity of Lawsonia intracellularis in Chinese herds.