论文部分内容阅读
目的：用羟基脲制造ＳＤ大鼠“阳虚”模型，以探讨其对体内代谢的影响。方法：３月龄雄性ＳＤ大鼠１６只，随机分为对照（Ａ）组和羟基脲（Ｂ）组，每组８只。Ａ组：灌喂生理盐水２ｍＬ／ｋｇ，每周６次；Ｂ组：灌喂羟基脲混悬液２．５ｇ／ｋｇ，每周３次。在处死大鼠前第１０天和第２天分别给其皮下注射盐酸四环素（２５ｍｇ／ｋｇ）。３个月后，取血清送中心实验室进行检测；取内脏称重；取胫骨中段行不脱钙骨包埋，锯片和磨片，再用半自动图象数字化分析仪（美国）对其皮质骨的骨组织作静态和动态测量及计算。结果：与对照组比较，羟基脲组的体重减少了１２．５％（Ｐ＜０．０５）；腓肠肌重量减少了１０％（Ｐ＜０．０５），内脏重量减轻，肝、肺和睾丸的重量分别减少了１２．５％（Ｐ＜０．０５），３２．８％（Ｐ＜０．０５）和４８．２％（Ｐ＜０．０５）；微量元素中Ｃｕ含量增加２２．９％（Ｐ＜０．０５），Ｔ４增加了８１．４％（Ｐ＜０．０５）；胫骨中段的皮质骨减少７％（Ｐ＜０．０５），骨髓腔增加２８％（Ｐ＜０．０１）。结论：羟基脲可使动物出现虚损表现和导致骨质疏松。
OBJECTIVE: To establish the “yang deficiency” model of SD rats with hydroxyurea to investigate its effect on metabolism in vivo. Methods: Sixteen 3-month-old male Sprague-Dawley rats were randomly divided into control (A) group and hydroxyurea (B) group, 8 rats in each group. Group A: saline 2mL / kg, 6 times a week; Group B: hydroxyurea suspension 2.5g / kg, 3 times a week. Tetracycline hydrochloride (25 mg / kg) was injected subcutaneously on the tenth day and the second day before the rats were sacrificed. After 3 months, take the serum to the center of the laboratory for testing; take visceral weighing; take the middle of the tibia with non-decalcified bone embedded, saw blades and grinding, and then semi-automatic image digitization analyzer (United States) on its cortex Bone and bone tissue for static and dynamic measurement and calculation. Results: Compared with the control group, the body weight of the hydroxyurea group decreased by 12.5% (P <0.05), the gastrocnemius muscle weight decreased by 10% (P <0.05), visceral weight loss, liver, lung and testicular (P <0.05), 32.8% (P <0.05) and 48.2% (P <0.05) respectively. The content of Cu in trace elements increased by 22.9% (P <0.05), T4 increased 81.4% (P <0.05), cortical bone decreased 7% (P <0.05) and bone marrow cavity increased 28% ). Conclusion: Hydroxyurea can make the animals appear wasting and lead to osteoporosis.