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目的探讨腹泻型肠易激综合征(D IBS)与IL10基因启动子区域-1082、-819和-592位点单核苷酸多态性之间的关系。方法用扩增受阻突变系统PCR方法对41例健康对照和43例D IBS患者IL10基因启动子区域-1082、-819和-592位点单核苷酸多态性进行研究。结果-819位点D IBS患者T/T基因型频率显著高于健康对照组(67.4%比39.0%,P<0.05),C/T和C/C基因型频率虽较对照组低(分别为23.3%比43.9%和9.3%比17.1%),但差异无统计学意义(P>0.05);-592位点D IBS患者A/A基因型频率显著高于对照组(67.4%比39.0%,P<0.05),C/A和C/C基因型频率均较对照组低(分别为23.3%比43.9%和9.3%比17.1%),但差异无统计学意义(P>0.05);-1082位点基因型频率差异无统计学意义(P>0.05)。IL10启动子-819位点T等位基因频率在D IBS患者显著高于健康对照组(79.1%比61.0%,P<0.05),C等位基因频率在D IBS患者显著低于健康对照组(20.9%比39.0%,P<0.05);-592位点A等位基因频率在D IBS患者显著高于对照组(79.1%比61.0%,P<0.05),C等位基因频率在D IBS患者显著低于对照组(20.9%比39.0%,P<0.05);-1082位点G或A等位基因频率在D IBS患者与对照组间差异无统计学意义。结论IL10基因启动子区域-819T/T和-592A/A基因型可能与D IBS发生有关。
Objective To investigate the relationship between diarrhea-predominant irritable bowel syndrome (D IBS) and single nucleotide polymorphisms at -1082, -819 and -592 in IL10 gene promoter region. Methods Single nucleotide polymorphisms at positions -1082, -819 and -592 of IL10 gene promoter in 41 healthy controls and 43 DIBS patients were studied by polymerase chain reaction-restriction fragment length polymorphism (PCR). Results The frequencies of T / T genotype in patients with D IBS at -819 sites were significantly higher than those in healthy controls (67.4% vs. 39.0%, P <0.05), while the frequencies of C / T and C / C genotypes were lower 23.3% vs 43.9% vs 9.3% vs 17.1%, respectively), but the difference was not statistically significant (P> 0.05). The frequency of A / A genotype in 592 DIBS patients was significantly higher than that in the control group (67.4% vs. 39.0% (P> 0.05). The frequencies of C / A and C / C genotypes were lower than those in the control group (23.3% vs 43.9% and 9.3% vs 17.1% respectively), but the difference was not statistically significant (P> 0.05) There was no significant difference in genotype frequencies (P> 0.05). The frequency of T allele at -819 locus in IL10 promoter was significantly higher in patients with D IBS than in healthy controls (79.1% vs 61.0%, P <0.05), and the frequency of C allele in D IBS patients was significantly lower than that in healthy controls 20.9% vs 39.0%, P <0.05). The frequency of allele A at position 592 was significantly higher in patients with D IBS than in controls (79.1% vs. 61.0%, P <0.05) (20.9% vs. 39.0%, P <0.05). The frequency of allele G or A at 1082 was not significantly different between D IBS patients and controls. Conclusions The -819T / T and -592A / A genotypes in IL10 gene promoter may be related to the occurrence of DIBS.