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为探讨He-Ne激光对紫外线-B(UV-B)辐射损伤修复途径,采用He-Ne激光辐照(5mW·mm-2)进行照射,对增强UV-B(13.08kJ·m-2·d-1)辐射下水稻幼苗的叶绿素含量、核酮糖-1,5-二磷酸羧化酶/加氧酶(rubisco)亚基含量及反映植物光系统II(PSII)的荧光参数值进行测定。结果表明,经UV-B处理后,水稻叶片中的叶绿素含量、rubisco亚基含量及荧光参数值(除qN以外)低于对照组(CK),差异显著(P<0.05),其中荧光参数Fv/Fm对环境胁迫反映最为敏感;而单独He-Ne激光(L)处理组均高于对照组(CK),差异不显著(P>0.05);经He-Ne激光和UV-B复合组(BL)均高于UV-B处理组,低于对照组,差异显著(P<0.05);说明UV-B辐射对水稻幼苗的光合系统有损伤作用,而一定剂量的He-Ne激光可以提高水稻的光合能力,表明He-Ne激光对增强UV-B辐射造成的损伤具有一定修复作用。
In order to explore the way of He-Ne laser to repair UV-B radiation damage, He-Ne laser irradiation (5mW · mm-2) d-1), the chlorophyll content, the content of Rubisco subunit and the fluorescence parameters of plant photosystem II (PSII) . The results showed that chlorophyll content, rubisco subunit content and fluorescence parameters (except qN) of rice leaves were significantly lower (P <0.05) after UV-B treatment, and the fluorescence parameters Fv / Fm was the most sensitive to environmental stress; while He-Ne laser alone (L) treatment group were higher than the control group (CK), the difference was not significant (P> 0.05); by He-Ne laser and UV-B complex group BL) were higher than that of UV-B treatment group and lower than that of control group (P <0.05), indicating that UV-B radiation could damage the photosynthetic system of rice seedlings, while a certain dose of He-Ne laser could increase rice Photosynthetic capacity, indicating that He-Ne laser to enhance the damage caused by UV-B radiation has some repair.