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目的：探讨蛋白激酶 D1（PKD1）对大鼠骨髓源性内皮祖细胞（EPCs）黏附、迁移、增殖、血管形成能力及内皮型一氧化氮合成酶（eNOS）表达的影响。方法体外培养、分离和鉴定大鼠骨髓源性 EPCs，观察 PKD1及其特异性阻断剂CID755673对 EPCs 的黏附、迁移、增殖、血管形成能力的影响，以及对 EPCs 中 eNOS 的 mRNA 表达和蛋白表达的影响。结果 EPCs 体外细胞培养实验表明，PKD1可明显促进EPCs 的黏附、迁移和增殖，提升 EPCs 的血管形成能力，上调EPCs 中 eNOS 的 mRNA 表达和蛋白表达水平。结论PKD1具有调控 EPCs 促血管新生的作用，其促血管新生的作用可能以一种依赖 eNOS 的方式进行。“,”Aim To explore the effect of protein kinase D1 (PKD1 )on adherence,migration,proliferation, microvascular formation,and eNOS expression in bone marrow-derived endothelial progenitor cells(EPCs)of rats.Method The EPCs were isolated from bonemarrow of rats,cultured and detected,the effects of PKD1 and its specific blocking agent CID755673 on adhesion,migration,proliferation,or microvascular formation were observed,as well as mRNA and protein expression of endothelial nitric oxide synthase (eNOS )in EPCs.Results PKD1 significantly promoted adhe-sion,migration,proliferation and microvascular forma-tion of EPCs,and upregulated the mRNA and protein expression of eNOS in EPCs,according to the cell cul-ture experiments of EPCs in vitro.Conclusion PKD1has the role of angiogenesis through regulation of EPCs,which might be dependent on eNOS.