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目的观察不同剂量辐射损伤对小鼠T淋巴细胞功能亚群的影响,探讨辐射所致的免疫系统损伤在细胞学和分子水平上的机制。方法C57BL/6j小鼠采用60Coγ射线照射诱导辐射损伤模型。总照射剂量分别为0.7、1.4、2.8、和5.6Gy。应用细胞表面标志和细胞内因子标记,流式细胞仪检测分析不同剂量组小鼠脾T淋巴细胞功能亚群CD3,CD4,CD8改变及Th1和Th2的变化。结果1.小鼠脾T-淋巴细胞功能亚群CD3,CD4和CD8在照射后均有明显降低,降低幅度与受照剂量明显相关。2.CD4/CD8比值显示照射后均有明显升高。3.照射后Th1和Th2均有明显降低,其中Th1降低明显高于Th2。受照剂量大于2.8Gy组Th2/Th1比值较空白对照组明显升高。结论不同照射剂量对T淋巴细胞功能亚群CD3,CD4,CD8和CD4/CD8有明显影响,Th1和Th2和Th2/Th1功能亚群的失衡在辐射所致的免疫损伤中具有较重要作用。
Objective To observe the effects of different doses of radiation injury on functional subsets of T lymphocytes in mice and to explore the mechanism of radiation-induced damage to the immune system at the cytology and molecular level. Methods C57BL / 6j mice were irradiated with 60Coγ ray to induce radiation injury. The total exposure dose was 0.7, 1.4, 2.8, and 5.6 Gy, respectively. Cell surface markers and intracellular factor markers were used to detect the changes of CD3, CD4, CD8 and Th1 and Th2 in splenic T lymphocyte subsets in different dose groups by flow cytometry. The results showed that the CD3, CD4 and CD8 subsets of splenic T-lymphocytes in mice were significantly decreased after irradiation, and the decreasing amplitude was significantly correlated with the dose of irradiation. CD4 / CD8 ratio showed significantly increased after irradiation. Th1 and Th2 after irradiation were significantly reduced, Th1 reduction was significantly higher than Th2. Compared with the blank control group, the Th2 / Th1 ratio in the irradiated dose greater than 2.8Gy group was significantly higher than that in the blank control group. Conclusion Different exposure doses have significant effects on the functional subsets CD3, CD4, CD8 and CD4 / CD8 of T lymphocytes. The imbalance of Th1 and Th2 / Th1 / Th1 functional subsets plays an important role in the radiation-induced immune damage.