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方法：经饮水摄入Ｎ－甲基－Ｎ′－亚硝基－Ｎ－亚硝胍（ＭＮＮＧ，１５０ｍｇＬ－１）制作大鼠胃腺癌模型，饲以１，２５－二羟基维生素Ｄ３〔１，２５（ＯＨ）２Ｄ３〕添加的饲料（２．５ｍｇｋｇ－１和５．０ｍｇｋｇ－１）。于实验１６、３２周处死动物，进行增生细胞核抗原（ＰＣ－ＮＡ）免疫组织化学和组织病理学研究。结果：实验１６周，１，２５（ＯＨ）２Ｄ３（５．０ｍｇｋｇ－１）添加组大鼠腺胃粘膜上皮细胞ＰＣＮＡ标记率明显高于只经饮水摄入ＭＮＮＧ组，实验３２周胃腺癌发生率、癌巢数和微血管密度显著增加。结论：一定剂量的１，２５（ＯＨ）２Ｄ３对ＭＮＮＧ诱发大鼠胃腺癌的发生有促进作用。
Methods: Rat model of gastric adenocarcinoma was induced by drinking N-methyl-N’-nitroso-N-nitrosoguanidine (MNNG, 150 mg L-1) and fed with 1,25-dihydroxyvitamin D3 25 (OH) 2D3] (2.5 mg kg-1 and 5.0 mg kg-1). Animals were sacrificed at 16 and 32 weeks of the experiment, and immunohistochemistry and histopathology of PC-NA were performed. Results: After 16 weeks of operation, the labeling rate of PCNA in glandular mucosa epithelial cells of 1,25 (OH) 2D3 (5.0mgkg-1) group was significantly higher than that of MNNG group only after drinking water for 24 weeks. The incidence of gastric adenocarcinoma , The number of cancer nests and microvessel density increased significantly. Conclusion: A certain dose of 1,25 (OH) 2D3 can promote the development of gastric adenocarcinoma in MNNG rats.