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用微波萃取技术从内蒙古黄芪[Astragalus membranaceus(Fisch.)Bunge var.mongholious(Bunge)Hsiao]的干燥根中提取黄芪多糖。依据响应面法(RSM,Response surface methodology)原理,使用Design Expert(Static Made Easy,Minneapolis,MN,USA.version6.0.5,2001)软件的中心组合设计建立试验数学模型,对微波萃取黄芪多糖的提取时间、加热温度、提取溶液pH值及液料比进行优化组合。得到最优回归方程:Y=1.14+1·258E-003X1+0.16X2-0.027X3+0.15X4+0.032X11-0.047X22+0.025X33-0.047X22+0.025X33+0.029X44+0.043X1X2-0.018X1X3-0.059X1X4-0.027X2X3+2.263E-003X2X4+0.012X3X4。确定最优操作工艺参数为:微波加热时间20min、加热温度120℃、液料比8∶1、pH值10。粗多糖得率为32%,纯度为44.4%。测定值与预测值相关系数r为0.976。
Astragalus polysaccharide was extracted from the dried roots of Astragalus membranaceus (Fisch.) Bunge var. Mongholious (Bunge) Hsiao in Inner Mongolia by microwave extraction. According to the principle of response surface methodology (RSM), a mathematical model of experiment was set up by using the center combination design of Design Expert (Static Made Easy, Minneapolis, MN, USA.version6.0.5, 2001) Time, heating temperature, extraction solution pH and liquid to material ratio to optimize the combination. The optimal regression equation is obtained: Y = 1.14 + 1.258E-003X1 + 0.16X2-0.027X3 + 0.15X4 + 0.032X11-0.047X22 + 0.025X33-0.047X22 + 0.025X33 + 0.029X44 + 0.043X1X2-0.018X1X3-0.059 X1X4-0.027X2X3 + 2.263E-003X2X4 + 0.012X3X4. Determine the optimum operating parameters: microwave heating time 20min, heating temperature 120 ℃, liquid material ratio 8: 1, pH value of 10. The crude polysaccharide yield was 32% with a purity of 44.4%. The correlation coefficient r of the measured value and the predicted value is 0.976.