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使用与胃癌相关的化学致癌剂Ｎ－甲基－Ｎ＇硝基－Ｎ－亚硝基胍（ＭＮＮＧ）对人类胃粘膜上皮细胞系ＧＥＳ－１进行恶性转化实验，得到转化后细胞ＭＣ。比较观察了ＧＥＳ－１和ＭＣ细胞的骨架形态。结果表明ＧＥＳ－１及ＭＣ，角蛋白在二细胞均为阳性，微管形态和分布无差异，而微丝肌动蛋白在ＧＥＳ－１细胞呈正常排列，ＭＣ细胞微丝出现明显的异常，显示了化学致癌剂ＭＮＮＧ对人胃粘膜细胞骨架的选择性作用的特点。本工作对深入研究亚硝酰胺类化学物质与胃癌发生的关系提供了一个细胞水平的线索。
Using gastric cancer-associated chemical carcinogen N-methyl-N’nitro-N-nitrosoguanidine (MNNG) to conduct malignant transformation on human gastric epithelial cell line GES-1 to obtain transformed MC. The skeleton morphology of GES-1 and MC cells was comparatively observed. The results showed that both GES-1 and MC were positive for keratin in both cells, and there was no difference in morphology and distribution of microtubules. Microfilament actin was normally arranged in GES-1 cells, and the microfilaments of MC cells showed obvious abnormalities. The selective action of chemical carcinogen MNNG on the cytoskeleton of human gastric mucosa. This work provides a clue at the cellular level for an in-depth study of the relationship between nitrosamines and gastric cancer.