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目的探讨二硫化碳对离体培养支持细胞凋亡及FasL基因表达的影响。方法以0、0．36、0．72、1．44μmol/ml浓度的二硫化碳作用于离体培养的大鼠支持细胞24 h后,应用噻唑蓝(MTT)法检测支持细胞存活率,流式细胞术检测细胞凋亡及逆转录聚合酶链反应(RT-PCR)技术检测凋亡相关基因FasL的表达。结果二硫化碳处理后的支持细胞存活率随作用浓度的增加而下降,当≥1．44μmol/ml浓度时,细胞存活率(73．34%±1．39%)明显下降,与溶剂对照组(99．98%±5．48%)比较,差异有统计学意义(P＜0．05)。随二硫化碳浓度升高,细胞凋亡率增加,当浓度≥1．44μmol/ml时,支持细胞凋亡率(7．93%±0．43%)明显升高,与溶剂对照组(3．63%±0．53%)比较,差异有统计学意义(P＜0．05)。随二硫化碳浓度增加,凋亡相关基因FasL表达量亦明显增加。结论二硫化碳对离体培养支持细胞具有细胞毒性作用,可导致细胞凋亡率升高,凋亡相关基因FasL高表达。
Objective To investigate the effects of carbon disulfide on the apoptosis of cultured somatotrophs and the expression of FasL gene. Methods After treated with carbon disulfide (0,0.36,0.72,1.44μmol / ml) for 24 h in vitro, the viability of supporting cells was measured by MTT assay. Flow cytometry Apoptosis was detected by surgery and reverse transcriptase polymerase chain reaction (RT-PCR) was used to detect the expression of FasL. Results The survival rate of CSCs treated with carbon disulfide decreased with the increase of the action concentration. The cell survival rate (73.34% ± 1.39%) was significantly decreased when the concentration of ≥1.44μmol / ml was increased. Compared with the solvent control group .98% ± 5.48%), the difference was statistically significant (P <0.05). With the concentration of carbon disulfide increased, the rate of apoptosis increased, when the concentration of ≥ 1.44μmol / ml, the rate of apoptosis (7.93% ± 0.43%) was significantly increased, compared with the solvent control group (3.63 % ± 0.53%), the difference was statistically significant (P <0.05). With the increase of carbon disulfide concentration, FasL expression of apoptosis related genes also increased significantly. Conclusion Carbon disulfide has a cytotoxic effect on cultured cells in vitro, which leads to the increase of apoptosis rate and the high expression of FasL.