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以西洋梨两年生植株的嫩茎为外植体材料，ＭＳ为基本培养基，当附加０．４ｍｇ／ＬＩＢＡ和０．６ｍｇ／Ｌ６－ＢＡ时，试管苗生长良好；生根培养基附加１．２ｍｇ／ＬＩＢＡ的生根效果最理想；开瓶炼苗４ｄ，闲瓶炼苗６ｄ的处理，试管苗移栽成活率高达９４．３％。镜检染色体数目２ｎ＝３４，细胞学性状稳定。
The tender shoots of two-year-old pears of Pear were planted as explants and MS as the basic medium. When 0.4mg / L IBA and 0.6mg / L 6-BA were added, the test tube seedlings grew well. The rooting medium supplemented with 1.2mg / The rooting effect of LIBA was the best. After 4 days of openning flask and 6 days of free bottle refining, the survival rate of transplanting in vitro seedlings was as high as 94.3%. Microscopy chromosome number 2n = 34, cytological stability.