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目的:观察卡波金(carbogen)对豚鼠急性噪声性声损伤后耳蜗外侧壁微血管的影响,探讨卡波金在声损伤早期治疗中的应用价值。方法:纯白健康豚鼠40只随机分为单纯噪声暴露组、单纯吸入卡波金组、噪声暴露加卡波金吸入组以及空白对照组。每组实验动物均为10只。应用活体显微镜技术,观察豚鼠噪声暴露和(或)吸入卡波金后耳蜗外侧壁微血管的变化情况,通过对微血管红细胞流柱宽度(RBC column diameter,RBC-CD)、血流速度(blood flow velocity,BFV)、血流流态(blood flow states,BFS)的描述反映耳蜗外侧壁局部微循环的变化。结果:空白对照组耳蜗外侧壁微血管血流稳定,单纯噪声暴露组可见方向相反的逆向血流,血管内可见成簇状的细胞聚集现象。与单纯噪声暴露组比较,噪声暴露加卡波金吸入组逆行血流减少。单纯吸入卡波金组RBCCD与对照组比较增加20.7%,单纯噪声暴露组较对照组RBCCD减少12.1%,组间差异有统计学意义(P<0.05)。噪声暴露加卡波金组较单纯噪声暴露组RBCCD增加17.4%。单纯吸入卡波金组血流呈线流或线粒流;空白对照组和噪声暴露加卡波金吸入组血流呈线粒流或粒线流。单纯噪声暴露组血流呈粒流、粒缓流甚至出现粒摆流。结论:吸入卡波金气体后耳蜗外侧壁血管RBCCD明显增加,并使血液流速加快。在急性声损伤的早期干预中,吸入卡波金对耳蜗微循环的改善是一种有积极意义的措施。
Objective: To observe the effect of carbogen on the outer wall of the cochlea after acute noise-induced acoustic guinea pig injury and to explore the application value of carbopol in the early treatment of acoustic injury. Methods: Forty pure white healthy guinea pigs were randomly divided into simple noise exposed group, inhaled carbopol group, noise exposed plus carbopol inhalation group and blank control group. Each group of experimental animals were 10. The change of microvascular in lateral wall of cochlea after noise exposure and / or inhalation of carbopol in guinea pigs was observed by using live microscope. The effects of RBC column diameter (RBC-CD), blood flow velocity , BFV), and blood flow states (BFS) reflect the changes in the local microcirculation of the cochlear lateral wall. Results: Microvessel blood flow was steady in the outer wall of cochlea in blank control group. Reverse flow in opposite direction was observed in simple noise exposure group, and clusters of cells clustered in blood vessels. Compared with simple noise exposure group, retrograde blood flow was decreased in noise exposure gacarbin inhalation group. Compared with the control group, RBCCD increased by 20.7% in the simple inhalation of carbopol group, and decreased by 12.1% in the noise-only exposure group compared with the control group. The difference was statistically significant (P <0.05). Noise exposure in the gabapentin group increased by 17.4% compared with the noise-exposed group. The blood flow of pure inhalation of carbopol group was linear or linear; the control group and noise exposure gabapentin inhalation group showed linear or granular flow. Pure noise exposure group showed a flow of grain flow, particle flow and grain flow or granule flow. CONCLUSIONS: RBCCD increases significantly on the outer lateral wall of the cochlea after inhaling carbopol gas and accelerates blood flow. In the early intervention of acute acoustic injury, inhaled carboplatin improves the microcirculation of the cochlea as a positive measure.