论文部分内容阅读
目的探讨血浆内毒素[主要成分为脂多糖(LPS)]和降钙素原(PCT)对败血症患者的诊断价值。方法血浆LPS的检测采用光度法,血浆PCT采用上转发光免疫分析仪进行检测,对222例临床确诊败血症患者和20健康体检者(对照组)的上述两项指标同时进行检测。结果败血症组LPS和PCT水平均高于对照组,差异有统计学意义(P<0.05)。败血症患者中,PCT阳性率为30.18%(67/222),PCT水平为(4.026±5.458)ng/mL。PCT检测阳性者67例,LPS水平为(0.307±0.238)EU/mL,LPS检测阳性率为94.03%(63/67)。败血症患者中,PCT检测阴性者占69.82%(155/222),PCT阴性者LPS水平为(0.102±0.114)EU/mL,LPS检测阳性率为19.35%(30/155),与PCT阳性者比较差异有统计学意义(P<0.05)。PCT用于败血症诊断的灵敏度和特异度分别为91.68%和94.56%,LPS的灵敏度和特异度分别为85.12%和64.76%。结论 LPS与PCT联合检测有利于提高革兰阴性菌感染检测的准确性,为临床诊断提供参考依据。
Objective To investigate the diagnostic value of plasma endotoxin (LPS) and procalcitonin (PCT) in patients with sepsis. Methods Plasma LPS was detected by spectrophotometry, and plasma PCT was detected by up-conversion immunoassay. The above two indexes of 222 clinically diagnosed sepsis patients and 20 healthy subjects (control group) were tested simultaneously. Results The levels of LPS and PCT in sepsis group were significantly higher than those in control group (P <0.05). In sepsis patients, the positive rate of PCT was 30.18% (67/222) and the PCT level was (4.026 ± 5.458) ng / mL. 67 cases were positive for PCT test, the level of LPS was (0.307 ± 0.238) EU / mL, and the positive rate of LPS was 94.03% (63/67). Among the sepsis patients, 69.82% (155/222) were negative for PCT test, (0.102 ± 0.114) EU / mL for PCT negative test, and 19.35% (30/155) for LPS test. Compared with PCT positive patients, The difference was statistically significant (P <0.05). The sensitivity and specificity of PCT for the diagnosis of sepsis were 91.68% and 94.56%, respectively. The sensitivity and specificity of LPS were 85.12% and 64.76% respectively. Conclusion The combination of LPS and PCT is beneficial to improve the accuracy of Gram-negative bacterial infection detection and provide a reference for clinical diagnosis.