【摘 要】
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To investigate the genetic bases of grain weight(GW),a set of 76 chromosome segment substitution lines(CSSLs,BC4F5)were developed from the cross between Xieqing
【机 构】
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State Key Laboratory of Rice Biology/National Center for Rice Improvement,China National Rice Resear
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To investigate the genetic bases of grain weight(GW),a set of 76 chromosome segment substitution lines(CSSLs,BC4F5)were developed from the cross between Xieqingzao B(XQZB)and Zhonghui 9308(ZH9308)using a marker-assisted selection(MAS).All substituted segments represented by 120 markers in those lines covered 96.7％of the donor parent(ZH9308).Consequently,two QTLs(qTGW1 and qTGW6)for 1000-grain weight(TGW)were preliminarily mapped on chromosomes 1 and 6,respectively.The line CSSL7 was selected for further mapping of qGL1.4/qTGW1.As a result,qGL1.4 was validated and narrowed to a 1.4 Mb interval between markers InDel15 and RM11872 using secondary F2,and narrowed to a 500 kb in F2∶3 populations between markers RM 11824 and RM 11842.In F4∶5 secondary population,using homozygous recombinant strategy,qGL1.4 was finally fine-mapped to a 286.4 kb region between markers D-12 and TG-57.In addition,a near-isogenic line(NIL)harbouring qGL1.4 was developed using MAS approach,which showed enhanced grain length compared with ZH9308 without changing other traits.In summary,these results lay a foundation for the genetic isolation of qGL1.4 and molecular breeding in rice.
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