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目的探讨乙酰血清素甲基转移酶(acetylserotonin methyltransferase,ASMT)基因启动子区的两个位点(rs4446909、rs5989681)多态性与卒中后睡眠障碍(post-stroke sleep disorders,PSSD)患者神经功能缺损程度的相关性。方法将2014年1月至2015年12月在我院就诊的395例首发脑卒中患者分为PSSD组和非PSSD组,使用PSQI量表评定睡眠质量,利用多重单碱基延伸SNP分型技术检测ASMT基因,并进行基因型和等位基因频率分布的统计分析,分析其与PSSD患者神经功能缺损程度的相关性。结果神经功能轻度缺损与中重度缺损相比:rs4446909、rs5989681位点各基因型及等位基因频率在两组间分布差异无统计学意义(P>0.05);PSSD组、非PSSD组中两多态性位点各基因型频率在轻度与中重度神经功能缺损组间的分布差异无统计学意义(P>0.05)。结论 ASMT基因启动子区rs4446909、rs5989681位点多态可能不是PSSD患者神经功能缺损程度的独立危险因素。
Objective To investigate the relationship between polymorphism of two loci (rs4446909, rs5989681) and post-stroke sleep disorders (PSSD) in acetylserotonin methyltransferase (ASMT) gene promoter in patients with neurological deficits Degree of relevance. Methods 395 primary stroke patients treated in our hospital from January 2014 to December 2015 were divided into PSSD group and non-PSSD group. The sleep quality was assessed by PSQI scale and detected by multiple single base extension SNP typing ASMT gene, and statistical analysis of genotype and allele frequency distribution, analysis of PSSD patients with neurological deficit degree correlation. Results There was no significant difference in the distribution of genotypes and alleles between rs4446909 and rs5989681 loci between the two groups (P> 0.05). There was no significant difference between the two groups in PSSD group and non-PSSD group There was no significant difference in the frequencies of genotypes between mild and moderate neurological deficit groups (P> 0.05). Conclusion The polymorphisms of rs4446909 and rs5989681 in ASMT gene promoter region may not be independent risk factors of neurological deficit in PSSD patients.