论文部分内容阅读
[目的]探讨内毒素腹腔注射染毒对大鼠肝组织抗氧化酶的影响。[方法]采用腹腔注射技术,以脂多糖(LPS)对Wister大鼠进行染毒,染毒浓度分别为0.01、0.05、0.10、1.00mg/kg,对肝组织匀浆上清液中的超氧化物歧化酶(SOD)、Cu,Zn-SOD酶、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-PX)活性以及谷胱甘肽(GSH)和丙二醛(MDA)含量进行测定。[结果]与对照组相比,染毒浓度分别为0.01、0.05和0.10mg/kg时,SOD酶活性变化无统计学差异(P>0.05);染毒浓度为1.00mg/kg时,有明显降低(P<0.01)。与对照组相比,Cu,Zn-SOD酶活性随着染毒浓度的增大,呈现先升后降的趋势,浓度达0.05mg/kg时,升高具有统计学意义(P<0.01)。与对照组相比,CAT酶活性和MDA含量的变化呈现先降后升的趋势,二者下降的最低点分别为0.05mg/kg和0.01mg/kg;GSH含量和GSH-PX活性均呈现先增后减的趋势,染毒浓度为0.05mg/kg时,二者的增高具有统计学意义(P<0.01)。[结论]低浓度的LPS可致大鼠肝组织产生适应性反应;高浓度的LPS可引起大鼠肝组织抗氧化能力降低,提示高浓度LPS对大鼠肝的毒作用可能与诱发体内产生过量自由基有关。
[Objective] To investigate the effect of intraperitoneal injection of endotoxin on anti-oxidative enzymes in rat liver tissue. [Method] The Wister rats were treated with lipopolysaccharide (LPS) by intraperitoneal injection. The concentrations of them were 0.01, 0.05, 0.10 and 1.00 mg / kg respectively. The supernatant of liver homogenate The activities of SOD, Cu, Zn-SOD, CAT, GSH-PX, glutathione (GSH) and malondialdehyde (MDA) ) Content was measured. [Result] Compared with the control group, SOD activity had no significant difference (P> 0.05) at the concentration of 0.01, 0.05 and 0.10 mg / kg respectively. When the concentration was 1.00 mg / kg, Decreased (P <0.01). Compared with the control group, the activity of Cu, Zn-SOD showed the trend of increasing first and then decreasing with the increasing of the concentration of the enzyme. When the concentration reached 0.05mg / kg, the activity of Cu and Zn-SOD increased statistically (P <0.01). Compared with the control group, CAT activity and MDA content showed a trend of decreasing firstly and then increasing, and the lowest decreasing point was 0.05mg / kg and 0.01mg / kg respectively; GSH content and GSH-PX activity showed the first Increase and then reduce the trend, when the exposure concentration of 0.05mg / kg, both increased statistically significant (P <0.01). [Conclusion] Low concentration of LPS can induce adaptive reaction in rat liver tissue. High concentration of LPS can reduce the antioxidant capacity of rat liver tissue, suggesting that the toxic effect of high concentration LPS on rat liver may be related to the excessive production of excessive Free radicals.