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通过检测分析,转Bt基因抗虫棉金杂棉3号是抗棉铃虫优良的新品种,在棉花不同的生育期,Bt杀虫蛋白含量幅度在94.2~896.8 ng.g-1,苗期、蕾期、花期的叶片Bt蛋白表达量级属于高表达,蕾期的小蕾和花期的花瓣Bt蛋白表达量级属于中高表达,花期的小蕾、铃期花瓣和小蕾Bt蛋白表达量级属于中表达,花期的小铃、铃期叶片和小铃Bt蛋白表达量级属于低表达。金杂棉3号的杀虫活性80.21%~91.87%,抗虫效率达83.33%~100.00%,保蕾效果达82.56%~100.00%。
Through testing and analysis, Bt insecticidal cotton Jinzamian 3 was an excellent new cotton bollworm resistant strain. The contents of Bt insecticidal protein ranged from 94.2 to 896.8 ng.g-1 at different growth stages of cotton, The expression level of Bt protein at the budding stage and the flowering stage was highly expressed. The expression level of Bt protein in the petals at the budding stage and the flowering stage was moderately high. The Bt protein expression level in the petals, The expression of small Bt, flowering stage bell and small bell Bt protein expression level is low expression. Jinzamian 3 insecticidal activity of 80.21% ~ 91.87%, insect-resistant efficiency of 83.33% ~ 100.00%, Bao Lei effect of 82.56% ~ 100.00%.