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目的观察大鼠失血性休克复苏后小肠杯状细胞在肠黏膜重建过程中的作用。方法SD大鼠49只,每只重250～300g,分实验和对照两组,实验组再分为6个组,分别为休克复苏后1、3、6、12、24和36h组;各组留取回肠黏膜,观察休克复苏后早期不同阶段肠黏膜的形态改变、杯状细胞数量变化和肠三叶因子-3(TFF3)的含量。结果休克后肠黏膜明显损伤,3h始肠黏膜出现损伤后修复现象,表现为杯状细胞聚集于损伤的肠黏膜表面;12h后大部分黏膜细胞被杯状细胞覆盖,杯状细胞呈过分泌现象;24h肠黏膜表面细胞连续性得到恢复。黏膜杯状细胞数量24h内从243±13下降到157±9,下降了35.4%(r=-0.910,P<0.01);36h组细胞数量明显回升,但仍低于对照组(t=4.01,P<0.01)。TFF3含量在12h前明显升高,24h有所下降,但仍高于对照组(t=3.24,P<0.05)。结论杯状细胞可能在肠黏膜损伤后的重建过程中起关键作用;TFF3的高表达可能是促进肠黏膜早期重建的重要因素。
Objective To observe the role of goblet cells in the reconstruction of intestinal mucosa after hemorrhagic shock and resuscitation in rats. Methods 49 Sprague-Dawley rats weighing 250-300g each were divided into experimental and control groups. The experimental group was divided into 6 groups: 1, 3, 6, 12, 24 and 36 h after shock resuscitation; The ileal mucosa was collected and the morphological changes of intestinal mucosa, the number of goblet cells and the content of intestinal trefoil factor-3 (TFF3) in different stages of shock resuscitation were observed. Results The intestinal mucosa was obviously damaged after shock, and the intestinal mucosa was repaired after 3 hours of injury. The goblet cells accumulated in the damaged intestinal mucosa surface. Most of mucosal cells were covered by goblet cells and goblet cells were over-secreted after 12 hours 24h intestinal mucosal cell surface recovery. Mucosal goblet cells decreased from 243 ± 13 to 157 ± 9 within 24 h, down 35.4% (r = -0.910, P <0.01). The number of cells in 36 h group was significantly increased but still lower than that in control group (t = 4.01, P <0.01). The level of TFF3 increased significantly before 12h and decreased 24h, but still higher than that of control group (t = 3.24, P <0.05). Conclusions Goblet cells may play a key role in the process of intestinal mucosal injury. High expression of TFF3 may be an important factor in the early reconstruction of intestinal mucosa.