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报道了基于金纳米棒表面增强拉曼散射(SERS)的免疫检测.将拉曼活性分子对巯基苯甲酸吸附于金纳米棒表面,制备出SERS标记的金纳米棒探针.该探针和蛋白抗体结合形成SERS标记抗体.通过SERS标记抗体、待测抗原和俘获抗体(固体基底上修饰的抗体,即俘获抗体)之间的免疫应答反应,将金纳米棒探针组装到固体基底上,形成SERS标记抗体-抗原-俘获抗体“三明治”夹心复合体.待测抗原浓度越大,固体基底上俘获的金纳米棒探针的数目越多,从而可通过SERS信号的强弱来检测待测抗原的浓度.由于金纳米棒的表面等离子体共振(SPR)峰位置可以在较宽的范围内调控,可通过激发光和SPR的耦合来提高SERS信号,从而提高免疫检测的灵敏度.单组分抗原可检出的浓度范围高于1×10-8mg/mL.
The immunoassay based on surface enhanced Raman scattering (SERS) of gold nanorods was reported, and the SERS-labeled gold nanorod probes were prepared by adsorbing the active thiol molecules onto the gold nanorods. The probe and protein Antibodies bind to form SERS-labeled antibodies Gold nanorod probes are assembled onto a solid substrate by the SERS-labeled antibody, the immune response between the test antigen and the capture antibody (modified antibody on the solid substrate, ie capture antibody), forming SERS labeled antibody - antigen - capture antibody sandwich sandwich.When the concentration of the antigen to be tested, the greater the number of gold nanorod probes captured on the solid substrate, which can be detected by the strength of the SERS signal To measure the concentration of antigens, the sensitivity of immunoassays can be improved by increasing the SERS signal by coupling the excitation light and the SPR, since the surface plasmon resonance (SPR) peak position of the gold nanorods can be controlled over a wide range. Antigen can be detected in the range of concentrations higher than 1 × 10-8mg / mL.