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通过对印度尼西亚蓝碧海峡3个澄黄滨珊瑚(Porites lutea)地理群体(YM、YN、YS)的79个样本核糖体RNA转录单元内间隔区ITS基因(包括ITS1区和ITS2区)片段进行克隆、测序,探讨澄黄滨珊瑚的群体遗传多样性和遗传分化.结果表明:79条序列共获得63个单倍型,3个群体呈现出高的单倍型多样性(Hd=0.991 56)和高的核苷酸多样性(Pi=0.026 81).Tajima’D检验、Fu’s Fs检验和岐点分布检验(mismatch analysis)推测种群经历了群体扩张事件.单倍型邻接关系树和单倍型网络图表明澄黄滨珊瑚群体没有明显的地理谱系结构.分子方差分析(AMOVA)表明群体间遗传变异占1.22%,群体内遗传变异占98.78%.群体间固定指数(Fst)的绝对值为0.012 2,表明群体间遗传分化较弱.各群体间的基因流值均大于4,说明澄黄滨珊瑚在当地群体间随机交配,基因交流十分充沛,各珊瑚群体间没有地理隔离,可以互相作为造礁珊瑚修复用的来源补充地.
The ITS gene (including ITS1 region and ITS2 region) of 79 internal ribosomal ribosomal RNA transcripts from 3 geographical populations (YM, YN, YS) of the Porites lutea community in the BlueBai strait of Indonesia was cloned , And sequenced to explore the population genetic diversity and genetic differentiation of Coral roots. The results showed that there were 63 haplotypes in 79 sequences and 3 haplotype diversity (Hd = 0.991 56) and (Pi = 0.026 81) .According to Tajima’D test, Fu’s Fs test and mismatch analysis, the population was experiencing population dilatation events.Hybrid haplotype adjacency trees and haplotype networks The results showed that no obvious geographical lineage structure was found in the coral population of Huangbin in China. The molecular variance analysis (AMOVA) showed that the genetic variation among the populations accounted for 1.22%, and the intraspecific genetic variation accounted for 98.78%. The absolute value of the Fst between groups was 0.012 2 , Indicating that the genetic differentiation between populations is weak.The genetic flow value of each group is greater than 4, indicating that the coral of Chenghuanghuang random mating among local groups, gene exchange is very abundant, there is no geographical isolation between the coral groups, can be mutually reef Coral repair used Additionally source.