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目的:探讨O型孕妇IgG抗A(B)效价和新生儿溶血3项对ABO新生儿溶血病(HDN)的预测及诊断价值.方法:选取2015-01—2016-12就诊于丹阳市人民医院妇产科的260例母婴血型不合的O型孕妇,产婴后185例为非HDN组,75例诊断为HDN,作为HDN组.运用2-巯基乙醇检测孕妇末次IgG抗A(B)效价,运用微柱凝胶免疫法检测新生儿溶血3项(直接抗人球蛋白试验、游离试验和放散试验).结果:与正常对照组相比,HDN组的IgG抗A(B)效价、直接抗人球蛋白试验、游离试验和放散试验及新生儿血型均差异有统计学意义(P＜0.05).对有差异的因素进行logistic回归分析,发现游离试验、放散试验对HDN的发生具有统计学意义(P＜0.05).通过ROC曲线分析,求出曲线下面积AUC,通过约登指数计算敏感性与特异性.虽然IgG抗A(B)效价对HDN的发生不具有预测价值(P = 0.312),但通过趋势x2检验发现,随着IgG抗A(B)效价的升高, HDN的发生率也显著增加(x2=31.63,P＜0.05).结论:游离试验和放散试验对HDN具有诊断价值,IgG抗A(B)效价对HDN不具有预测价值,但IgG抗A(B)效价升高会导致HDN的发生概率增加.“,”Objective: To investigate the value of maternal IgG anti-A(B)titres and neonatal hemolysis three trials in predicting and diagnosing ABO hemolytic disease of newborn(HDN). Method: A retrospective analysis of 260 cases of maternal IgG anti-A(B) titres and neonatal hemolysis three trials in predicting and diagnosing ABO HDN. Result:IgG anti-A(B)titres,neonatal hemolysis three trials(direct antiglobulin test,free antibodies tese and elution test)and blood type were statistically different between control group and HDN group(P＜0.05). Logistic regression analysis was carried out on these factors,free antibodies test and elution test had positive diagnostic value for HDN(P＜0.05). Sensitivity and specificity were calculated through ROC curves. Although IgG anti-A(B)ti-tres didn\'t have predictive value for the development of HDN(P = 0.312),but we found that as IgG anti-A(B)tit-res increased,the incidence of HDN also increased significantly(x2 = 31.63,P＜0.05). Conclusion:Free antibodies test and elution test had positive diagnostic value for HDN. Although IgG anti-A(B)titres didn\'t have predictive value for the development of HDN,as IgG anti-A(B)titres increased,the incidence of HDN also increased significantly.