论文部分内容阅读
利用氯酸盐对串珠镰刀菌菌株进行诱变并获得nit突变体 (硝酸盐利用缺陷突变体 ) ,所获得突变体中 ,以nitA比例最高。采用nit突变体互补型配对技术 ,测定分离自水稻、玉米、棉花及大豆植物上串珠镰刀菌的营养体亲和群。结果显示 ,1 5 7个菌株分属于 71个营养体亲和群 ;不同群体间的菌株仅测出玉米田群体内的一个菌株 (YM0 5 )和大豆田群体内的两个菌株 (D0 8和D1 2 )属于同一个营养体亲和群 ,其它群体间的菌株则分属不同的营养体亲和群。菌株SA1 8经抗药性诱变、连续转代培养和在不同温度下保存半年处理 ,菌株的营养体亲和性可发生改变 ;单孢株后代的营养体亲和性也可发生改变
Mutants of Fusarium moniliforme strains were mutagenized with chlorate and nit mutants (nitrate utilization deficient mutants) were obtained. Among the mutants obtained, the highest proportion of nitA was obtained. Mutant pairing of nit mutants was used to determine vegetative compatibility of Fusarium moniliforme isolated from rice, corn, cotton and soybean plants. The results showed that 157 isolates belonged to 71 vegetative affinities; only one isolates (YM0 5) and two isolates from soybean population (D0 8 and D1 2) belong to the same nutrient body affinity group, strains of other groups belong to different nutrient body affinity group. The strain SA1 8 was resistant to mutagenesis, successive subcultures and stored at different temperatures for six months, the vegetative compatibility of the strains can be changed; the vegetative affinity of the offspring of single spore strains can also change