构建内皮细胞三维芽生模型,为调控肿瘤血管生成药物的药效研究提供一种更为准确的检测手段。通过倒挂悬滴式的培养方法使脐静脉内皮细胞形成致密的细胞团,再将细胞团分别放入Matrigel,鼠尾胶及纤维胶3种基质中进行三维培养。结果显示内皮细胞团在纤维胶中形成了明显的相互连接的毛状管腔样结构,即芽生。经验证,内皮细胞团在纤维胶中能形成明显的管腔样结构,且该方法能够成为一种体外观察研究管腔形成的有效方法。 To build a three-dimensional endothelial cell budding model for the regulation of tumor angiogenesis drug efficacy studies provide a more accurate detection means. The hanging cell culture was used to form a dense cell mass in the umbilical vein endothelial cells. The cell mass was placed into three kinds of matrigel, rat tail glue and fibrin glue respectively for three-dimensional culture. The results showed that the endothelial cell mass formed obvious interconnected hairy lumen-like structures in the gums, ie budding. It has been proved that the endothelial cell mass can form obvious luminal structure in the fibrin glue and the method can be an effective method for observing the lumen formation in vitro.