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表面污染和材料褐变是龙眼组织离体培养成活率低的主要原因，采用切取中间茎段内部组织培养和多种药液交替浸泡的前处理方法，是降低污染率、褐化率，成功建立无菌初代培养物的有效措施；不同物候期的茎段，诱芽率不同，以夏、秋季的茎段较为适合作为起始外植体；选用MS基本培养基诱芽，诱芽率可达73％左右，且生长整齐，叶色较绿，为以脱毒为目的的龙眼茎尖培养研究提供无菌、无褐变的茎尖。
Surface contamination and material browning are the main reasons for the low survival rate of longan tissue in vitro. The pretreatment method of cutting tissue culture in intermediate stem section and alternating immersion of multiple chemicals is to reduce the contamination rate and browning rate and establish successfully Aseptic primary culture of effective measures; different stages of the phenology of the stem, the rate of different buds, summer and autumn stems more suitable as the starting explant; selection MS basic medium induced buds up to the rate of up to 73% or so, and growing neatly, the leaf color is green, for the purpose of detoxification longan shoot tip culture to provide sterile, non-browning shoot tips.