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毛细管电泳是一种在生命科学应用最为广泛的生物分子分离技术,目前已广泛应用于DNA测序,SNP检测,DNA片段分析等。筛分介质是毛细管电泳性能的关键因素,筛分介质的种类、结构、分子量及所形成的溶液决定着生物分子的迁移特性、分辨率、读出长度、重现性等参数,对分离结果有重大影响。本文对近年来用于DNA分离的无胶筛分介质的研究和应用情况进行综述,希望能对相关研究和鉴定提供参考和借鉴。
Capillary electrophoresis is a biomolecular separation technique widely used in life sciences. It has been widely used in DNA sequencing, SNP detection and DNA fragment analysis. Screening medium is the key factor of capillary electrophoresis performance. The type, structure, molecular weight of the sieving medium and the solution formed determine the migration characteristics, resolution, read length, reproducibility and other parameters of the biomolecule. Tremendous influence. This review summarizes the research and application of gel-free screening media for DNA separation in recent years, and hopes to provide reference and reference for related research and identification.