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目的:制定女贞子总三萜酸的质量标准。方法:对11批不同产地的女贞子药材进行性状鉴别、薄层色谱鉴别,测定其杂质、水分、总灰分和浸出物的含量;应用HPLC-UV,建立女贞子中总三萜酸含量的测定方法,按照10倍量70%乙醇,每次0.5 h将11批女贞子药材的总三萜酸提取完全,合并提取液,浓缩,测定含量。结果:杂质、总灰分和浸出物的含量均符合《中华人民共和国药典》规定,部分批次药材含水量稍高;女贞子总三萜酸以齐墩果酸计,含量不得低于0.95%。结论:2010版《中华人民共和国药典》一部中并没有对女贞子三萜酸含量做出明确规定,本研究对女贞子总三萜酸的质量标准做出补充,为女贞子三萜酸的进一步开发奠定基础。
Objective: To develop the quality standard of total triterpene acid in Ligustrum lucidum. METHODS: The traits of Fructus Ligustri Lucidi from 11 batches of different provenances were identified by TLC. The contents of impurity, water, total ash and extract were determined. The content of total triterpene acid in Fructus Ligustri Lucidi was determined by HPLC-UV , The total triterpene acids of 11 batches of Fructus Ligustri Lucidae were extracted completely according to 10 times the amount of 70% ethanol for 0.5 h. The extracts were combined and concentrated to determine the content. Results: The contents of impurity, total ash and extract were in accordance with the Pharmacopoeia of the People’s Republic of China. Some batches of herbs had slightly higher water content. The total triterpene acid in Fructus Ligustri Lucidi was determined to be not less than 0.95% . CONCLUSION: The 2010 version of “Pharmacopoeia of the People’s Republic of China” does not specify the content of triterpene acid in Ligustrum lucidum. In this study, the quality of Ligustrum lucidum total triterpene acid was supplemented, The further development of terpene acid laid the foundation.