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目的:研究黄芩苷对流感病毒FM1感染小鼠肺组织NO及氧化损伤的保护作用。方法:将96只ICR鼠随机分为正常对照组、模型组、利巴韦林组(100mg.kg-1.d-1),黄芩苷大剂量组(1 500mg.kg-1.d-1),黄芩苷中剂量组(750mg.kg-1.d-1)和黄芩苷小剂量组(375mg.kg-1.d-1),每组16只。用流感病毒亚甲型鼠肺适应株A/FM/1/47(H1N1)感染小鼠,建立小鼠流感病毒性肺炎的生物模型,用不同剂量黄芩苷溶液灌胃治疗后,观察小鼠肺指数、肺组织H-E染色切片病理形态变化,比色法检测肺组织匀浆总NO合成酶(NOS)、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-PX)含量或活性。结果:黄芩苷中、小剂量组均能明显降低肺指数,减轻肺组织病变;显著下调肺匀浆总NO合成酶、丙二醛及上调谷胱甘肽过氧化物酶含量或活性(P<0.05,P<0.01)。结论:黄芩苷可通过抑制流感病毒性肺炎小鼠肺组织NO及过氧化物水平而发挥保护作用。
Objective: To study the protective effect of baicalin on lung tissue NO and oxidative damage induced by influenza virus FM1. Methods: 96 ICR mice were randomly divided into normal control group, model group, ribavirin group (100mg.kg-1.d-1), high dose of baicalin group (1500mg.kg-1.d-1 ), Baicalin middle dose group (750mg.kg-1.d-1) and baicalin low dose group (375mg.kg-1.d-1), 16 in each group. The mouse model of influenza virus pneumonia was established by inoculating mice with influenza A virus FMDV A / FM / 1/47 (H1N1). After treatment with different doses of baicalin solution, the mice lung Index, pathological changes of lung tissue HE staining sections, the content of total NO synthase (NOS), malondialdehyde (MDA), glutathione peroxidase (GSH-PX) or activity . Results: Both baicalin and low dose groups could significantly reduce the lung index and alleviate the lung pathological changes; significantly reduce the content of total NO synthase, malondialdehyde and glutathione peroxidase in lung homogenate (P < 0.05, P <0.01). Conclusion: Baicalin can play a protective role by inhibiting NO and peroxide levels in lung tissue of mice with influenza virus pneumonia.