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目的 利用仓鼠胰腺癌模型研究大网膜淋巴管状斑在胰腺癌播散、转移中的意义。方法 用PGHAM 1细胞系建立胰腺癌侵犯胰腺被膜动物模型共 15只。第 7、14和 2 1天各剖杀 5只 ,肉眼、光学显微镜下观察癌细胞播散、入侵和转移的形态学改变。结果 模型仓鼠网膜上的管状斑于第 7天显示微转移灶 ;壁层腹膜的间皮细胞开始脱落。第 14天显示多个较大的微转移灶 ;壁层腹膜的间皮细胞大片脱落 ,癌细胞粘附到裸露的基底膜上 ;第 2 1天微转移灶增大 ,肉眼观察到腹腔肿大的淋巴结 ;癌细胞于壁层腹膜原位增殖 ,未侵透基底膜。结论 大网膜淋巴管状斑在胰腺癌早期腹腔播散、淋巴结转移和可能的血行转移中有重要意义。
Objective To study the significance of lymphatic tube spots in pancreatic cancer in the dissemination and metastasis of pancreatic cancer using hamster pancreatic cancer model. Methods A total of 15 pancreatic cancer invading pancreatic capsule models were established using PGHAM 1 cell line. On the 7th, 14th, and 21st day, 5 mice were sacrificed. Morphological changes of dissemination, invasion, and metastasis of cancer cells were observed under light microscope. Results Microscopic metastases appeared on the tubular plaque on the hamster omentum on the 7th day; mesothelial cells in the parietal peritoneum started to fall off. On the 14th day, multiple large micrometastases were visualized; large sections of mesothelial cells in the parietal peritoneum were detached, and cancer cells adhered to the bare basement membrane; micrometastases increased on the 2nd day, and abdominal enlargement was observed with the naked eye. Lymph nodes; cancer cells proliferate in situ in the parietal peritoneum without penetrating the basement membrane. Conclusion The omental lymphatic tube plaque plays an important role in early peritoneal dissemination, lymph node metastasis and possible hematogenous metastasis of pancreatic cancer.