家兔桑椹胚在1.5 mol/L的DMSO中被快速冷冻并在液氮(-196℃)中保存。以500℃/min复温,回收到的144个胚胎中形态正常者113个(78.47％)。将其中66个胚胎于复温后立即移植给8个受体兔,4只妊娠(50％)。胚胎植入率为16.7％,产仔率为9.1％。体外培养的29个胚胎、26个发育到胚泡期。应用双醋酸荧光素实验(FDA)对复温后胚胎活力进行判断,方法简便,快速精确。 Rabbit morula was rapidly frozen in 1.5 mol / L DMSO and stored in liquid nitrogen (-196 ° C). At 500 ℃ / min rewarming, 113 embryos (78.47%) were normal in 144 embryos recovered. Sixty-six embryos were transplanted immediately after rewarming to eight recipient rabbits and four pregnancies (50%). Embryo implantation rate was 16.7% and litter size was 9.1%. Twenty-nine embryos cultured in vitro developed to the blastocyst stage. Application of fluorescein acetate test (FDA) to determine the vitality of embryos after rewarming, the method is simple, fast and accurate.