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目的:以老龄大鼠为研究对象,观察左旋丁苯酞(L-3-n-Butylphthalide,1-NBP)对脑低灌注大鼠学习记忆的改善作用,并研究其皮质及海马β-淀粉样肽(Aβ1-42)水平变化。方法:永久性结扎老龄大鼠双侧颈总动脉3个月,制备脑低灌注模型。实验分4组:假手术组、溶剂对照组、30 mg·kg~(-1)-NBP和120 mg·kg~(-1)-NBP组。1-NBP组连续给予1-NBP 45 d后,用Morris水迷宫检测大鼠的空间学习和记忆以及保存记忆的能力。同时,取大鼠脑皮质及海马组织,用ELISA法检测Aβ1-42含量,以探讨1-NBP改善认识功能作用机制。结果:1-NBP可以显著缩短脑低灌注大鼠找到安全台的潜伏期,并明显增加脑低灌注大鼠在目标象限活动时间的百分比,明显降低大鼠皮质及海马Aβ1-42含量。结论:1-NβP能改善脑低灌注大鼠的学习记忆缺陷,减少皮质及海马Aβ1-42的水平,这可能是改善脑低灌注大鼠认知功能的重要机制之一。
OBJECTIVE: To observe the effect of L-3-n-Butylphthalide (1-NBP) on learning and memory of rats with cerebral hypoperfusion and to investigate the effects of beta-amyloid in cortex and hippocampus Changes in peptide (Aβ1-42) levels. Methods: Bilateral common carotid arteries were permanently ligated in aged rats for 3 months to prepare hypoperfusion model. The experiment was divided into 4 groups: sham operation group, solvent control group, 30 mg · kg -1 -NBP and 120 mg · kg -1 -NBP group. After 1-NBP administration in 1-NBP group for 45 days, Morris water maze was used to detect spatial learning and memory and memory ability of rats. At the same time, take the rat cerebral cortex and hippocampus tissue, and detect the content of Aβ1-42 by ELISA to explore the mechanism of 1-NBP improving cognitive function. Results: 1-NBP significantly shortened the latency of finding a safe platform in rats with hypoperfusion and markedly increased the percentages of activity time in the target quadrant of cerebral hypoperfusion rats, and significantly decreased the content of Aβ1-42 in the cortex and hippocampus of rats. CONCLUSION: 1-NβP can improve the learning and memory deficits and decrease the level of Aβ 1-42 in the cortex and hippocampus of rats with cerebral hypoperfusion, which may be one of the important mechanisms to improve the cognitive function in rats with hypoperfusion.