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尽管在垂直的电场和流体场作用下,采用芯片自由流电泳(μ-FFE)可实现样品的连续微分离和制备,但是由于在运行过程中,存在分析物的区带展宽问题,会直接影响样品的分离效果.在本文中,在施加固定电压的情况下,通过向和分离缓冲液相同的电极缓冲液中添加硫酸钠的方法,在分离腔内形成了梯度电场.通过对罗丹明B和甲基绿混合物的分离发现,在均一电场下,施加400V分离电压,混合物需2min才能完全分离;甲基绿的区带宽度为3.8mm,与罗丹明B的分辨率是3.2.在向电极缓冲液中添加5mmol/L硫酸钠形成的电场梯度下,施加300V的分离电压,两种染料可在10s内完成分离;在20s时,甲基绿的区带宽度被压缩到0.5mm,检测灵敏度提高了7倍以上;与罗丹明B的分辨率可达到16.2.此外,该方法还被用于牛血清白蛋白的富集.与施加均一电场相比,蛋白质的检测灵敏度得到了显著提高.上述结果表明,通过在μ-FFE中引入梯度电场,可有效提高样品的分辨率、检测灵敏度和分析速度.
Although continuous microfluidic separation and preparation of samples using chip free-flow electrophoresis (μ-FFE) under vertical electric fields and fluid fields, there is a direct effect on the band broadening of the analyte during operation In this paper, a gradient electric field is formed in the separation chamber by adding sodium sulfate to the same electrode buffer as the separation buffer under the application of a fixed voltage, The separation of the methyl green mixture showed that under a uniform electric field, a voltage of 400 V was applied and the mixture required 2 minutes to completely separate; the band width of methyl green was 3.8 mm and the resolution with rhodamine B was 3.2. Under the electric field gradient formed by adding 5 mmol / L sodium sulfate to the solution, a separation voltage of 300 V was applied and the two dyes could be separated within 10 s. At 20 s, the band width of methyl green was compressed to 0.5 mm and the detection sensitivity was improved More than 7 times; with rhodamine B resolution of up to 16.2. In addition, the method was also used for the enrichment of bovine serum albumin, compared with the application of a uniform electric field, the detection sensitivity of the protein has been significantly improved above results table By introducing an electric field gradient in the μ-FFE, can effectively improve the resolution of the sample, the detection sensitivity and speed of analysis.