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目的建立活络油中有效成分α-蒎烯、桉油精、樟脑、薄荷脑、水杨酸甲酯、桂皮醛、丁香酚、麝香草酚的气相色谱测定法,为该制剂的质量控制提供参考。方法采用气相色谱法,色谱条件以PEG-20M毛细管柱(30 m×0.25 mm×0.25μm),程序升温:起始温度80℃,保持3 min,每分钟升15℃,终止温度180℃,保持10 min,分流比50:1,FID检测器。结果线性范围:α-蒎烯0.50~7.54 mg·mL~(-1),桉油精0.51~7.59 mg·mL~(-1),樟脑0.16~2.44 mg·mL~(-1),薄荷脑0.67~10.01 mg·m L~(-1),水杨酸甲酯1.17~17.54 mg·mL~(-1),桂皮醛0.16~2.47 mg·mL~(-1),丁香酚0.34~5.04 mg·mL~(-1),麝香草酚0.17~2.51 mg·mL~(-1),回归方程的相关系数在0.99994~0.99998之间。结论本研究所采用的气相色谱法操作快速、简便、准确,精密度、重复性、稳定性好,可用于活络油的质量控制。本方法也可适用于含有类似成分的药油的检测和质量评价。
OBJECTIVE To establish a gas chromatographic method for the determination of α-pinene, eucalyptol, camphor, menthol, methyl salicylate, cinnamaldehyde, eugenol and thymol in active oil. . Methods Gas chromatography was used. The chromatographic conditions were as follows: PEG-20M capillary column (30 m × 0.25 mm × 0.25 μm), temperature programmed temperature of 80 ℃ for 3 min, temperature of 15 ℃ per minute and temperature of 180 ℃ 10 min, split ratio 50: 1, FID detector. Results Linear range: 0.50 ~ 7.54 mg · mL -1 of α-pinene, 0.51 ~ 7.59 mg · mL -1 of eucalyptol, 0.16 ~ 2.44 mg · mL -1 of camphor, 0.67 ~ 10.01 mg · m L -1, methyl salicylate 1.17 ~ 17.54 mg · mL -1, cinnamic aldehyde 0.16 ~ 2.47 mg · mL -1, eugenol 0.34 ~ 5.04 mg · ML -1 and thymol 0.17 ~ 2.51 mg · mL -1 .The correlation coefficients of regression equation ranged from 0.99994 to 0.99998. Conclusion The gas chromatography used in this study is fast, simple, accurate, accurate, reproducible and stable, and can be used for the quality control of active oil. The method can also be applied to the detection and quality evaluation of medicinal oils containing similar components.