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采用离体培养的人皮肤成纤维细胞并加入巨噬细胞调理培养液（MCM），及3H-胸腺嘧啶掺入液体闪烁检测，观察了成纤维细胞受MCM刺激后的增殖及药物对其的抑制作用。结果，加入MCM组细胞增殖率较对照组明显增加（P＜0．01），150ng／ml道诺霉素及联合用药组的细胞抑制率最高达95％以上（P＜0．01），250μg／ml去炎松的抑制率在不加MCM组约为20％～40％，而在加MCM组约为40％～60％（P＜0．01）。证实去炎松除有直接抑制作用外，更能明显抑制巨噬细胞活性因子对成纤维细胞增生的刺激作用。
Human dermal fibroblasts cultured in vitro were added with macrophage conditioned medium (MCM) and 3H-thymidine incorporation into liquid scintillation assay. The proliferation of fibroblasts stimulated by MCM and the inhibition by the drugs were observed effect. As a result, the cell proliferation rate in MCM group was significantly higher than that in control group (P <0.01). The cell inhibitory rate of 150ng / ml daunorubicin and combination group was up to 95% (P <0.01) / Ml, the inhibition rate of triamcinolone is about 20% ~ 40% in the group without MCM and 40% ~ 60% in the group with MCM (P <0.01). Confirmed that except for the direct inhibition of inflammation, but also significantly inhibited the stimulation of macrophage activation factor on fibroblast proliferation.