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目的探讨红益胶囊对-10°头低位卧床28 d诱导的猕猴下肢骨骼肌萎缩的保护作用,开发红益胶囊在肌萎缩防护中的新用途。方法采用-10°头低位卧床制备猕猴模拟失重性肌萎缩模型;猕猴分为对照组和喂食红益胶囊组,分别在卧床前(0天)和卧床后28 d取一侧比目鱼肌,应用免疫组织化学和实时定量PCR等方法,分析红益胶囊对肌萎缩形成的影响。结果 1)卧床28 d后,猕猴小腿平均围径和比目鱼肌肌纤维平均直径较卧床前显著下降(P<0.05),直径较小肌纤维含量明显增多,提示-10°头低位卧床28 d明显诱导了猕猴下肢骨骼肌和肌纤维的萎缩。2)卧床28 d后,猕猴比目鱼肌肌纤维横截面积明显下降,但红益胶囊组下降幅度较对照组显著减少(P<0.01)。3)-10°头低位卧床28 d后,肌萎缩特异因子Atrogin-1 mRNA的表达显著增加,红益胶囊组Atrogin-1 mRNA的表达受到了明显抑制(P<0.05)。结论红益胶囊可通过抑制肌萎缩特异因子Atrogin-1 mRNA的表达对抗-10°头低位卧床诱导的肌萎缩。提示了红益胶囊在肌萎缩防护中的新功能。
Objective To explore the protective effect of Hongyi Capsule on skeletal muscle atrophy of the lower limbs of macaque monkey induced by -10 ° head-down bed for 28 days, and to develop a new use of Hongyi Capsule in the prevention and treatment of muscular atrophy. METHODS: A model of simulated weightlessness muscular atrophy in macaques was established by -10 ° head-down bed. The rhesus monkeys were divided into control group and Feiyi Hongyi Capsule group. One side of soleus (0 day) Histochemistry and real-time quantitative PCR methods to analyze Hongyi capsule on muscle atrophy. Results 1) After 28 days of bed rest, the mean diameter of the calf and the average diameter of the muscle fibers in the soleus decreased significantly (P <0.05), and the diameter of the smaller fibers increased significantly. Rhesus monkey atrophy of skeletal and muscle fibers of the lower limbs. 2) After 28 days of bed rest, the cross-sectional area of muscle fibers in the soleus muscle decreased significantly, but the decrease in the Hongyi capsule group was significantly lower than that in the control group (P <0.01). 3) After 28 days of -10 ° head-down ambulation, Atrogin-1 mRNA expression was significantly increased, and Atrogin-1 mRNA expression was significantly inhibited in Hongyi Capsule group (P <0.05). Conclusion Hongyi Capsule can prevent muscular atrophy induced by ambulatory -10 ° head-down hypothesis by inhibiting the expression of Atrogin-1 mRNA. Prompted Hongyi capsule in the prevention and treatment of muscular atrophy new features.