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以叉鞭金藻为材料进行了有机磷农药——久效磷的毒性实验.结果表明,在久效磷胁迫下,微藻的生长受到了严重地抑制(72hEC_(50)= 3.49mg/L);其过氧化物酶(POD)活性降低,膜脂过氧化产物丙二醛(MDA)含量和电解质外渗率同步增加;超氧化物歧化酶(SOD)活性时而上升,时而下降.培养液中维生素C(V_tC)和维生素E(V_tE)的加入在一定程度上可缓解藻细胞的伤害.而核黄素(维生素B_2,简称V_tB_2)的加入可加重藻细胞的伤害.前者使电解质外渗率降低,后者则使电解质外渗率增加.显然,自由基参与了久效磷对细胞的伤害;而POD比SOD在清除叉鞭金藻细胞自由基中起着重要的作用.初步认为细胞的自由基伤害是久效磷抑制叉鞭金藻生长的原因之一.
The toxicity test of organophosphorus pesticide - monocrotophos was carried out with the species of Chokela, the growth of microalgae was severely inhibited under monocrotophos (72hEC 50 = 3.49mg / L ), The activity of peroxidase (POD) decreased, the content of membrane lipid peroxidation product (MDA) and electrolyte leakage rate increased synchronously, and the activity of superoxide dismutase (SOD) The addition of vitamin C (V_tC) and vitamin E (V_tE) can alleviate the damage of algae cells to a certain degree, while the addition of riboflavin (vitamin B_2, V_tB_2 for short) can aggravate the damage of algae cells.The former makes electrolyte extravasation The latter is to make the electrolyte leakage rate increased.Obviously, the free radicals involved in the monocrotophos on cell damage; and POD than SOD in the clearance of free radical sclaterism Chrysomeloid plays an important role.Preliminary that the cells Of free radical damage is one of the reasons for the inhibition of monocrotophos growth.