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目的建立高特异性﹑高敏感性可分离及鉴别人体尿液中巴比妥类药物的程序方法。方法①用Toxi-tube提取人体尿液中的N,N-二甲基甲酰胺二甲基缩醛衍生物(DMF-DMA)。②运用GC/MS﹑SIM系统及药品定量软件系对人体尿液中巴比妥类药物:布他比妥,异戊巴比妥,戊巴比妥,司巴比妥,苯巴比妥进行定性定量分析。结果①GC/MS对人体尿液中司巴比妥最低检出浓度为30 ng/mL,对布他比妥﹑异戊巴比妥﹑戊巴比妥﹑苯巴比妥最低检出浓度为200 ng/ml。②在实验的运行操作中该方法 /程序显示很高的精确度和准确度。结论此检测方法选择性好﹑检测简便﹑灵敏﹑定性定量结果准确,可用于巴比妥类药物中毒及巴比妥类药物滥用的临床检验诊断。
OBJECTIVE To establish a high specificity, high sensitivity procedure for the isolation and identification of barbiturates in human urine. Methods ① Toxi-tube extraction of N, N-dimethylformamide dimethyl acetal derivative (DMF-DMA) from human urine. ② The use of GC / MS ﹑ SIM system and the Department of Quantitative Drugs on the human urine in the barbiturates: burtarobi, amobarbital, pentobarbital, pentobarbital, phenobarbital Qualitative and quantitative analysis. Results ①GC / MS had the lowest detection limit of 30 ng / mL for sibutubin in human urine and 200 for bupivirtin, pentobarbital, pentobarbital and phenobarbital ng / ml. ② The method / procedure shows high accuracy and accuracy in experimental operation. Conclusion The method has the advantages of good selectivity, simple and sensitive detection, accurate qualitative and quantitative results, and can be used in the clinical diagnosis of barbiturate poisoning and barbiturate abuse.