论文部分内容阅读
诊断对虾病原的方法有传统的形态病理学 (光镜和电镜直接观察和组织病理学等 )、扩增和生物测定、微生物学和血清学方法。对皮下及造血组织坏死病毒病 (IHHNV)、肝胰脏细小病毒病 (HPV)、拖拉症 (Taurasyndrome ,TS)、白斑综合症 (WSSV)、斑节对虾型杆状病毒病 (MBV)和杆状对虾病毒病 (BP)等病原均采用非放射性的基因组探针。目前已研制出了NHP、某些弧菌 (Vibriospp)和微孢子虫的传统基因探针。根据聚合酶链式反应 (PCR) ,采用DNA扩增方法确立的检测某些病原的高敏感性方法也应用在对虾病原诊断上
Methods for the diagnosis of shrimp pathogens are traditional morphological pathology (light and electron microscopy direct observation and histopathology, etc.), amplification and bioassay, microbiology and serological methods. Immunohistochemical staining for subcutaneous and hematopoietic necrosis virus (IHHNV), hepatopancreas parvovirus (HPV), Taurasyndrome (TS), white spot syndrome (WSSV), Penaeus monodon (MBV) The shrimp virus disease (BP) and other pathogens are using non-radioactive genomic probe. NHP, some of the traditional gene probes for Vibriospp and Microsporidia have been developed. According to the polymerase chain reaction (PCR), the highly sensitive method of detecting certain pathogens established by DNA amplification method is also applied to the diagnosis of shrimp pathogens