在模拟生理条件下,用荧光光谱法研究间苯三酚与人血清白蛋白在缓冲溶液中、不同温度下的相互作用,以及黄体酮对其的影响。根据荧光猝灭双倒数图计算间苯三酚和人血清白蛋白之间的结合常数和结合位点数。结果显示,间苯三酚与人血清白蛋白之间为静态猝灭,且两者的结合常数和结合位点数均随黄体酮浓度的增大而增大。黄体酮可延长间苯三酚在人体内的作用时程。 Fluorescence spectroscopy was used to study the interaction of phloroglucinol and human serum albumin in buffer solution at different temperatures and the effects of progesterone on them under simulated physiological conditions. The binding constants and binding sites between phloroglucinol and human serum albumin were calculated according to double-reciprocal fluorescence quenching. The results showed that there was static quenching between phloroglucinol and human serum albumin, and both of the binding constants and binding sites increased with the increase of progesterone concentration. Progesterone prolongs the action of phloroglucinol in the human body.